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GARS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GARS Antibody (C-term) AW5648-U200
    All lanes : Anti-GARS Antibody (C-term) at1:2000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: C6 whole cell lysate Lane 5: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - GARS Antibody (C-term) AW5648-U200
    All lanes : Anti-GARS Antibody (C-term) at1:4000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P41250
Other Accession Q5RBL1
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW H=83;M=82;R=72 KDa
Additional Information
Gene ID 2617
Antigen Region 706-739 aa
Other Names Glycine--tRNA ligase, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS
Dilution WB~~1:4000
Target/Specificity This GARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-739 amino acids from the C-terminal region of human GARS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GARS
Function Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.
Cellular Location Cytoplasm. Mitochondrion. Cell projection, axon Note=Associated with granules in cultured neuron cells
Tissue Location Widely expressed, including brain and spinal cord.
Citations (0)

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Background

GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This protein is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. The protein has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

References

Shiba K., Schimmel P.J. Biol. Chem. 269:30049-30055(1994) Antonellis A., Ellsworth R.E.Am. J. Hum. Genet. 72:1293-1299(2003)

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$ 325.00
$ 110.00
Cat# AW5648-U200
Size:
Quantity:
(40 western blots)
Availability: In Stock
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