Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   beta II Tubulin Antibody   

beta II Tubulin Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Products labeled with a "crown" meet aggressive quality standards. Learn more.
  • FC - beta II Tubulin Antibody AW5682-U200
    Overlay histogram showing NIH/3T3 cells stained with AW5682 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5682, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • WB - beta II Tubulin Antibody AW5682-U200
    All lanes : Anti-beta II Tubulin at1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: human brain lysate Lane 4: human Kidney lysate Lane 5: NIH/3T3 whole cell lysate Lane 5: mouse lung lysate Lane 5: PC12 whole cell lysate Lane 5: rat liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7TMM9
Other Accession P09203, Q9NFZ7, Q13885, Q4R5B3, P85108, Q6B856, Q9BVA1, Q9CWF2, Q3KRE8, P32882, P13602, Q9NFZ5, P30156, P20802, O59837, P02554
Reactivity Human, Mouse, Rat
Predicted Human, Mouse, Monkey, Dog, Sheep, Chicken
Host Rabbit
Clonality Polyclonal
Calculated MW M=50;H=50;R=50 KDa
Additional Information
Gene ID 22151
Antigen Region 30-46 aa
Other Names Tubulin beta-2A chain, Tubb2a, Tubb2
Dilution FC~~1:25
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 14-46 amino acids from human.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsbeta II Tubulin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tubb2a
Synonyms Tubb2
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).
Cellular Location Cytoplasm, cytoskeleton.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).


Carninci P.,et al.Science 309:1559-1563(2005).
Lubec G.,et al.Submitted (JAN-2009) to UniProtKB.
Janke C.,et al.Science 308:1758-1762(2005).
Rogowski K.,et al.Cell 137:1076-1087(2009).
Yoshida K.,et al.Biochem. Biophys. Res. Commun. 389:506-511(2009).

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

$ 315.00
$ 110.00
Cat# AW5682-U200
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions