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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   PD L1 Monoclonal Antibody (PDL1)   

PD L1 Monoclonal Antibody (PDL1)

Purified Mouse Monoclonal Antibody (Mab)

     
  • WB - PD L1 Monoclonal  Antibody (PDL1) AW5698
    All lanes : Anti-PDL-1 Antibody at 0.5-1µg/ml dilution Lane 1: 293T/PDL1 whole cell lysate Lane 2: 293T whole cell lysate Lane 3: NCI-H1975 whole cell lysate Lane 4: MDA-MB231 whole cell lysate Lane 5: MCF-7 whole cell lysate Lane 6: NCI-H226 whole cell lysate Lysates/proteins at 30 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L),Peroxidase conjµgated at 1/5000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698
    Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by abgent test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698
    Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Dako test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698
    Immunohistochemical analysis of PDL-1 in human non-small cell lung carcinoma sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Leica test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698
    Immunohistochemical analysis of PDL-1 in human tonsil tissue sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by abgent test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698
    Immunohistochemical analysis of PDL-1 in human tonsil tissue sections(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) by Dako test. Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698
    Immunohistochemical analysis of PDL-1 in MCF-7 cell (left) and NCI-H226(right) cell sections by abgent test . Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698
    Immunohistochemical analysis of PDL-1 in untransfected(left) or transfected(right) with 293T cell sections by abgent test . Cell was fixed with formaldehyde; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (0.85µg/ml) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • IHC-P - PD L1 Monoclonal  Antibody (PDL1) AW5698
    AW5698 staining PD-L1 in human lung squamous carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • FC - PD L1 Monoclonal  Antibody (PDL1) AW5698
    Overlay histogram showing isotype control(blue line), 293T-PDL1(green line), MDA-MB-231(red line), NCI-H226(black line) cells stained with PD L1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NH174309) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession Q9NZQ7
Reactivity Human
Predicted Human
Host Mouse
Clonality monoclonal
Isotype IgG1
Clone Names 1929CT160.162.147
Calculated MW 33275 Da
Additional Information
Gene ID 29126
Other Names Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1
Target/Specificity This PD L1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 256-290 amino acids from the human region of human PD L1.
Dilution WB~~0.5-1µg/ml
IHC-P~~1:100
FC~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPD L1 Monoclonal Antibody (PDL1) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD274 (HGNC:17635)
Function Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).
Cellular Location Cell membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Recycling endosome membrane; Single-pass type I membrane protein. Note=Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation. Isoform 2: Endomembrane system; Single-pass type I membrane protein
Tissue Location Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Research Areas
Citations (0)

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Background

Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

References

Dong H.,et al.Nat. Med. 5:1365-1369(1999).
Freeman G.J.,et al.J. Exp. Med. 192:1027-1034(2000).
He X.-H.,et al.Acta Pharmacol. Sin. 26:462-468(2005).
Chi X.-Y.,et al.Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 400.00
Cat# AW5698
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(40 western blots)
Availability: In Stock
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