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DANRE efnb2a Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DANRE efnb2a Antibody (Center) Azb10031a
    Anti-(DANRE) efnb2a Antibody (Center) at 1:1000 dilution + ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - DANRE efnb2a Antibody (Center) Azb10031a
    DANRE efnb2a Antibody (Center) (Cat. #Azb10031a) western blot analysis in zebra fish heart tissue lysates (35ug/lane).This demonstrates the DANRE efnb2a antibody detected the DANRE efnb2a protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O73874
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36724 Da
Antigen Region 163-194 aa
Additional Information
Gene ID 30219
Other Names Ephrin-B2a, efnb2a, efnb2
Target/Specificity This DANRE efnb2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-194 amino acids from the Central region of DANRE efnb2a.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDANRE efnb2a Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name efnb2a
Synonyms efnb2
Function Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Research Areas
Citations (0)

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Background

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration (By similarity).

References

Durbin L., et al. Genes Dev. 12:3096-3109(1998).
Chan J., et al. Dev. Biol. 234:470-482(2001).

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$ 295.00
$ 99.00
Cat# Azb10031a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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