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DANRE atg4b Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - DANRE atg4b Antibody (N-term) Azb10037a
    All lanes : Anti-(DANRE) atg4b Antibody (N-term) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - DANRE atg4b Antibody (N-term) Azb10037a
    DANRE atg4b Antibody (N-term) (Cat. #Azb10037a) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the DANRE atg4b antibody detected the DANRE atg4b protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6DG88
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44453 Da
Antigen Region 1-30 aa
Additional Information
Other Names Cysteine protease ATG4B, 3422-, Autophagy-related protein 4 homolog B, atg4b, apg4b
Target/Specificity This DANRE atg4b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of DANRE atg4b.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDANRE atg4b Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name atg4b
Synonyms apg4b
Function Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE). Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity).
Cellular Location Cytoplasm.
Research Areas
Citations (0)

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Cysteine protease required for autophagy, which is able to cleave the C-terminal part of proteins that may be subsequently converted to a smaller form, with a revealed C-terminal glycine, considered to be the phosphatidylethanolamine (PE)-conjugated form. This conjugated form has the capacity for the binding to autophagosomes (By similarity).

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$ 295.00
$ 99.00
Cat# Azb10037a
(40 western blots)
Availability: In Stock
Bulk Size
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