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> home > Products > Primary Antibodies > Antibody Collections > Zebrafish > DANRE atg4b Antibody (N-term)
DANRE atg4b Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q6DG88 |
| Reactivity | Zebrafish |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 44453 Da |
| Antigen Region | 1-30 aa |
| Other Names | Cysteine protease ATG4B, 3422-, Autophagy-related protein 4 homolog B, atg4b, apg4b |
|---|---|
| Target/Specificity | This DANRE atg4b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of DANRE atg4b. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DANRE atg4b Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | atg4b {ECO:0000250|UniProtKB:Q9Y4P1} |
|---|---|
| Function | Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. Required for canonical autophagy (macroautophagy), non- canonical autophagy as well as for mitophagy. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy. Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q9Y4P1}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9Y4P1}. Cytoplasmic vesicle, autophagosome {ECO:0000250|UniProtKB:Q9Y4P1}. Endoplasmic reticulum {ECO:0000250|UniProtKB:Q9Y4P1}. Mitochondrion {ECO:0000250|UniProtKB:Q9Y4P1}. Note=Mainly localizes to the cytoplasm, including cytosol. {ECO:0000250|UniProtKB:Q9Y4P1} |
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Cysteine protease required for autophagy, which is able to cleave the C-terminal part of proteins that may be subsequently converted to a smaller form, with a revealed C-terminal glycine, considered to be the phosphatidylethanolamine (PE)-conjugated form. This conjugated form has the capacity for the binding to autophagosomes (By similarity).
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$ 365.00
$ 140.00
Cat# Azb10037a
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