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hsp90a.1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - hsp90a.1 Antibody (Center) Azb18696c
    All lanes : Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution Lane 1: Zebrafish muscle lysate Lane 2: Zebrafish lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - hsp90a.1 Antibody (Center) Azb18696c
    Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution + ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - hsp90a.1 Antibody (Center) Azb18696c
    All lanes : Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution Lane 1: Zebrafish muscle lysate Lane 2: Zebrafish lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - hsp90a.1 Antibody (Center) Azb18696c
    Immunohistochemical analysis of paraffin-embedded Z. skeletal muscle section using hsp90a. 1 Antibody (Center)(Cat#Azb18696c). Azb18696c was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • WB - hsp90a.1 Antibody (Center) Azb18696c
    Western blot analysis of lysate from zebra fish heart tissue lysate, using (DANRE) hsp90a. 1 Antibody (Center) (Cat. #Azb18696c). Azb18696c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q90474
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB47443
Calculated MW 83319 Da
Additional Information
Gene ID 30591
Other Names Heat shock protein HSP 90-alpha 1, hsp90a1, hsp90, hsp90a, hsp90aa1
Target/Specificity This hsp90a.1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 229-263 amino acids from the Central region of human (DANRE) hsp90a.1.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionshsp90a.1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name hsp90a.1
Synonyms hsp90, hsp90a, hsp90aa1
Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity). Plays a key role in slow and fast muscle development in the embryo. Plays a role in myosin expression and assembly.
Cellular Location Melanosome. Cytoplasm, myofibril, sarcomere, Z line Cytoplasm, myofibril, sarcomere, A band. Cytoplasm, perinuclear region. Note=Expressed at the Z line and in the perinuclear region of myofibrils. Shuttles between the Z line and A band in response to stress conditions and fibril damage
Tissue Location Strongly expressed in the early embryos within the somitic slow muscle progenitors, the adaxial cells that lie on either side of the notochord but not the notochord. Also expressed during the early differentiation of fast fibers. Detected in developing cardiac muscles and pectoral fin primordia. Not detected in mature muscle fibers.
Research Areas
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Background

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity). Plays a key role in slow and fast muscle development in the embryo. Plays a role in myosin expression and assembly.

References

Lele Z.,et al.Dev. Biol. 210:56-70(1999).
Etard C.,et al.Dev. Biol. 308:133-143(2007).
Etard C.,et al.J. Cell Biol. 180:1163-1175(2008).
Howe K.,et al.Nature 496:498-503(2013).
Krone P.H.,et al.Biochem. Biophys. Res. Commun. 204:746-752(1994).

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$ 295.00
$ 99.00
Cat# Azb18696c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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