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(DANRE) gapdh Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (DANRE) gapdh Antibody (C-term) Azb18698c
    Western blot analysis of lysate from HUVEC cell line, using (DANRE) gapdh Antibody (C-term)(Cat. #Azb18698c). Azb18698c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q5XJ10
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB46516
Calculated MW 35784 Da
Additional Information
Gene ID 317743
Other Names Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, gapdh {ECO:0000312|ZFIN:ZDB-GENE-030115-1}
Target/Specificity This (DANRE) gapdh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 273-298 amino acids from the C-terminal region of human (DANRE) gapdh.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(DANRE) gapdh Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name gapdh {ECO:0000312|ZFIN:ZDB-GENE-030115-1}
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).
Cellular Location Cytoplasm, cytosol. Nucleus. Cytoplasm, cytoskeleton
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Background

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).

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$ 295.00
$ 99.00
Cat# Azb18698c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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