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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Zebrafish   >   (DANRE) mylipb Antibody (C-term)   

(DANRE) mylipb Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (DANRE) mylipb Antibody (C-term) Azb18718c
    Western blot analysis of lysates from zebra fish brain, zebra fish muscle, Zebrafish tissue lysate (from left to right), using (DANRE) mylipb Antibody (C-term)(Cat. #Azb18718c). Azb18718c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q05AK5
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Clone Names RB47051
Additional Information
Other Names E3 ubiquitin-protein ligase MYLIP-B, 632-, Myosin regulatory light chain-interacting protein B, MIR-B, mylipb {ECO:0000312|ZFIN:ZDB-GENE-061027-67}
Target/Specificity This DANRE mylipb antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 440-475 amino acids from the C-terminal region of DANRE mylipb.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(DANRE) mylipb Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name mylipb {ECO:0000312|ZFIN:ZDB-GENE-061027-67}
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC). Regulates cell movements during gastrulation by acting downstream of fz7 to antagonize the frizzled-signaling pathway (By similarity).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q6TEM9} EMBL; BC124450; AAI24451.1; -; mRNA RefSeq; NP_001073491.1; NM_001080022.2 UniGene; Dr.74694; - ProteinModelPortal; Q05AK5; - SMR; Q05AK5; - PaxDb; Q05AK5; - GeneID; 565911; - KEGG; dre:565911; - CTD; 565911; - ZFIN; ZDB-GENE-061027-67; mylipb HOGENOM; HOG000007353; - HOVERGEN; HBG052549; - InParanoid; Q05AK5; - KO; K10637; - PhylomeDB; Q05AK5; - UniPathway; UPA00143; - PRO; PR:Q05AK5; - Proteomes; UP000000437; Unplaced GO; GO:0005737; C:cytoplasm; ISS:UniProtKB GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central GO; GO:0007369; P:gastrulation; ISS:UniProtKB GO; GO:0030178; P:negative regulation of Wnt signaling pathway; ISS:UniProtKB CDD; cd14473; FERM_B-lobe; 1 Gene3D; 1.20.80.10; -; 1 Gene3D; 2.30.29.30; -; 1 Gene3D; 3.30.40.10; -; 1 InterPro; IPR019749; Band_41_domain InterPro; IPR014352; FERM/acyl-CoA-bd_prot_sf InterPro; IPR035963; FERM_2 InterPro; IPR019748; FERM_central InterPro; IPR000299; FERM_domain InterPro; IPR018979; FERM_N InterPro; IPR018980; FERM_PH-like_C InterPro; IPR011993; PH-like_dom_sf InterPro; IPR029071; Ubiquitin-like_domsf InterPro; IPR001841; Znf_RING InterPro; IPR013083; Znf_RING/FYVE/PHD Pfam; PF00373; FERM_M; 1 Pfam; PF09379; FERM_N; 1 PRINTS; PR00935; BAND41 SMART; SM00295; B41; 1 SMART; SM01196; FERM_C; 1 SUPFAM; SSF47031; SSF47031; 1 SUPFAM; SSF54236; SSF54236; 1 PROSITE; PS50057; FERM_3; 1 PROSITE; PS50089; ZF_RING_2; 1 2: Evidence at transcript level; Complete proteome; Cytoplasm; Developmental protein; Gastrulation; Metal-binding; Reference proteome; Transferase; Ubl conjugation pathway; Zinc; Zinc-finger CHAIN 1 464 E3 ubiquitin-protein ligase MYLIP-B /FTId=PRO_0000365452 DOMAIN 1 279 FERM. {ECO:0000255|PROSITE- ProRule:PRU00084} ZN_FING 381 416 RING-type. {ECO:0000255|PROSITE- ProRule:PRU00175} SEQUENCE 464 AA; 52661 MW; C9B99363E62D7C54 CRC64; MLCHITRPDS VVLEVEVDPK ANGEDILNKI CQKMGIIEVD YFGLQFTGTK GESLWMNLRN RICQEVDCVS PCRLRLRVKF FVEPHLILQE QTRHLFLMHV KEEIIKGSLR LDAEQAIELC ALLAQAEFGD YKQNTAKYCY SQIYGQDPSH DTINTISLKH KSLEGVSQAS AEYQALQLVS SLTYYGVEWH FARDSEGQQL LIGVGQEGLF VCKSDFTPIE RLMYPVIQMA TQSGRNVYVT ITKDNGDSVV LLFKFVSPSA ANGLYRAITE IHAFYRCDTV MSTVKMQYSR DFKGHLASLF LNESIDLGKR YIFDIQRTSK EVYDRTRRAL FNAGVSVNGR GISRSLLRQT KVDREERMCV DCRETHVLKE KLQRLQEALT CALCCEQEIS AAFCPCGHMF CCYNCASQLQ CCPVCRSEVD RVQHVYLPTC ASLLGLAEAK TTNSVLRRTG ISEDCANKEN ARQM
Research Areas
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Background

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC). Regulates cell movements during gastrulation by acting downstream of fz7 to antagonize the frizzled-signaling pathway (By similarity).

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$ 295.00
$ 99.00
Cat# Azb18718c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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