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AUH Antibody (C-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q13825 |
|---|---|
| Other Accession | NP_001689.1 |
| Clone Names | 90121076 |
| Gene ID | 549 |
|---|---|
| Other Names | Methylglutaconyl-CoA hydratase, mitochondrial, AU-specific RNA-binding enoyl-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AUH |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | AUH |
|---|---|
| Function | Catalyzes the fifth step in the leucine degradation pathway, the reversible hydration of 3-methylglutaconyl-CoA (3-MG-CoA) to 3- hydroxy-3-methylglutaryl-CoA (HMG-CoA) (PubMed:12434311, PubMed:16640564, PubMed:11738050, PubMed:12655555). Can catalyze the reverse reaction but at a much lower rate in vitro (PubMed:16640564). HMG-CoA is then quickly degraded by another enzyme (such as HMG-CoA lyase) to give acetyl-CoA and acetoacetate (PubMed:16640564). Uses other substrates such as (2E)-glutaconyl-CoA efficiently in vitro, and to a lesser extent 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA), crotonyl-CoA ((2E)-butenoyl-CoA) and 3-hydroxybutanoyl-CoA (the missing carboxylate reduces affinity to the active site) (PubMed:16640564). Originally it was identified as an RNA-binding protein as it binds to AU-rich elements (AREs) in vitro (PubMed:7892223). AREs direct rapid RNA degradation and mRNA deadenylation (PubMed:7892223). Might have itaconyl-CoA hydratase activity, converting itaconyl-CoA into citramalyl-CoA in the C5-dicarboxylate catabolism pathway (PubMed:29056341). The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, an antimicrobial metabolite and immunomodulator produced by macrophages during certain infections, that can act as a vitamin B12-poisoning metabolite (PubMed:29056341). |
| Cellular Location | Mitochondrion {ECO:0000250|UniProtKB:Q9JLZ3}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The methylglutaconyl-CoA hydratase, mitochondrial proteinbinds to the AU-rich element (ARE), a common element found in the3' UTR of rapidly decaying mRNA such as c-fos, c-myc andgranulocyte/ macrophage colony stimulating factor. ARE elements areinvolved in directing RNA to rapid degradation and deadenylation.AUH is also homologous to enol-CoA hydratase, an enzyme involved infatty acid degradation, and has been shown to have intrinsichydratase enzymatic activity. AUH is thus a bifunctional chimerabetween RNA binding and metabolic enzyme activity. A possiblesubcellular localization in the mitochondria has been demonstratedfor the mouse homolog of this protein which shares 92% identitywith the human protein. It has been suggested that AUH may have anovel role as a mitochondrial located AU-binding protein. Human AUHis expressed as a single mRNA species of 1.8 kb, and translated asa 40-kDa precursor protein which is subsequently processed to a32-kDa mature form.
References
Kurimoto, K., et al. Proteins 75(2):360-372(2009)Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)Mack, M., et al. FEBS J. 273(9):2012-2022(2006)Illsinger, S., et al. Pediatr. Neurol. 30(3):213-215(2004)Ly, T.B., et al. Hum. Mutat. 21(4):401-407(2003)
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