|Other Names||tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, MHsaIIP, PuMet, TRDMT1, DNMT2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1021c was selected from the DNMT2 region of human TRDMT1 (DNMT2). A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).|
|Tissue Location||Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. TRDMT1 shows similarity to DNA methyltransferases, but this protein does not display methyltransferase activity. The protein strongly binds DNA, suggesting that it may mark specific sequences in the genome.
Hermann, A., et al., J. Biol. Chem. 278(34):31717-31721 (2003).Franchina, M., et al., Int. J. Biochem. Cell Biol. 33(11):1104-1115 (2001).Dong, A., et al., Nucleic Acids Res. 29(2):439-448 (2001).Yoder, J.A., et al., Hum. Mol. Genet. 7(2):279-284 (1998).Van den Wyngaert, I., et al., FEBS Lett. 426(2):283-289 (1998).
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