CLLD8/SETDB2 Antibody (Center V514) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q96T68 |
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Clone Names | 4020610 |
Gene ID | 83852 |
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Other Names | Histone-lysine N-methyltransferase SETDB2, Chronic lymphocytic leukemia deletion region gene 8 protein, Lysine N-methyltransferase 1F, SET domain bifurcated 2, SETDB2, C13orf4, CLLD8, KMT1F |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP1075b was selected from the Center region of human SETDB2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | SETDB2 |
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Synonyms | C13orf4, CLLD8, KMT1F |
Function | Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere- associated repeats. Plays a role in chromosome condensation and segregation during mitosis. |
Cellular Location | Nucleus. Chromosome |
Tissue Location | Ubiquitous. Highest expression in heart, testis and ovary |
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Provided below are standard protocols that you may find useful for product applications.
Background
Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity.
References
Zhang, Y., et al., Nat. Genet. 34(2):181-186 (2003).Mabuchi, H., et al., Cancer Res. 61(7):2870-2877 (2001).
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