|Other Names||Ras association domain-containing protein 5, New ras effector 1, Regulator for cell adhesion and polarization enriched in lymphoid tissues, RAPL, RASSF5, NORE1, RAPL|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Note=Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A|
|Tissue Location||Widely expressed. Frequently down-regulated in lung tumor cell lines and primary lung tumors|
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The protein encoded by this gene is highly similar to thegene product of Schizosaccharomyces pombe rad17, a cell cyclecheckpoint gene required for cell cycle arrest and DNA damagerepair in response to DNA damage. This protein shares strongsimilarity with DNA replication factor C (RFC), and can form acomplex with RFCs. This protein binds to chromatin prior to DNAdamage and is phosphorylated by the checkpoint kinase ATR followingdamage. This protein recruits the RAD1-RAD9-HUS1 checkpoint proteincomplex onto chromatin after DNA damage, which may be required forits phosphorylation. The phosphorylation of this protein isrequired for the DNA-damage-induced cell cycle G2 arrest, and isthought to be a critical early event during checkpoint signaling inDNA-damaged cells. Eight alternatively spliced transcript variantsof this gene, which encode four distinct proteins, have beenreported. Two pseudogenes, located on chromosomes 7 and 13, havebeen identified.
Zhang, L., et al. EMBO J. 29(10):1726-1737(2010)Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)Beretta, G.L., et al. Cancer Lett. 266(2):194-202(2008)Zhao, M., et al. Head Neck 30(1):35-42(2008)Rodriguez-Bravo, V., et al. Cancer Res. 66(17):8672-8679(2006)
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