|Other Names||Histone-lysine N-methyltransferase SETD2, HIF-1, Huntingtin yeast partner B, Huntingtin-interacting protein 1, HIP-1, Huntingtin-interacting protein B, Lysine N-methyltransferase 3A, SET domain-containing protein 2, hSET2, p231HBP, SETD2, HIF1, HYPB, KIAA1732, KMT3A, SET2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1196b was selected from the C-term region of human SET2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||HIF1, HYPB, KIAA1732, KMT3A, SET2|
|Function||Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha-TubK40me3) (PubMed:27518565). Alpha- TubK40me3 is required for normal mitosis and cytokinesis and is proposed to be a specific tag in cytoskeletal remodeling (PubMed:27518565).|
|Cellular Location||Nucleus. Chromosome|
|Tissue Location||Ubiquitously expressed.|
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SET2 is a histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. This protein probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. SET2 binds DNA at promoters, and may act as a transcription activator. SET2 binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. SET2 belongs to a class of huntingtin interacting proteins characterized by WW motifs.
Rega, S., et al., Mol. Cell. Neurosci. 18(1):68-79 (2001).Passani, L.A., et al., Hum. Mol. Genet. 9(14):2175-2182 (2000).Faber, P.W., et al., Hum. Mol. Genet. 7(9):1463-1474 (1998).
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