|Other Names||E3 ubiquitin-protein ligase RNF5, 632-, Protein G16, RING finger protein 5, Ram1 homolog, HsRma1, RNF5, G16, NG2, RMA1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||G16, NG2, RMA1|
|Function||Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.|
|Cellular Location||Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane Note=Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene contains a RING finger,which is a motif known to be involved in protein-proteininteractions. This protein is a membrane-bound ubiquitin ligase. Itcan regulate cell motility by targeting paxillin ubiquitination andaltering the distribution and localization of paxillin in cytoplasmand cell focal adhesions.
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :Tcherpakov, M., et al. J. Biol. Chem. 284(18):12099-12109(2009)Zhong, B., et al. Immunity 30(3):397-407(2009)McKinnon, E., et al. Diabetes Obes Metab 11 SUPPL 1, 92-100 (2009) :Bromberg, K.D., et al. Cancer Res. 67(17):8172-8179(2007)
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