|Other Names||Poly(A) polymerase gamma, PAP-gamma, Neo-poly(A) polymerase, Neo-PAP, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme, PAPOLG, PAP2, PAPG|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13183b was selected from the C-term region of PAPOLG. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Responsible for the post-transcriptional adenylation of the 3'-terminal of mRNA precursors and several small RNAs including signal recognition particle (SRP) RNA, nuclear 7SK RNA, U2 small nuclear RNA, and ribosomal 5S RNA.|
|Tissue Location||Expressed predominantly in testis, and weakly in other tissues. Overexpressed in several tumors|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the poly(A) polymerasefamily which catalyzes template-independent extension of the 3' endof a DNA/RNA strand. This enzyme shares 60% identity to the wellcharacterized poly(A) polymerase II (PAPII) at the amino acidlevel. These two enzymes have similar organization of structuraland functional domains. This enzyme is exclusively localized in thenucleus and exhibits both nonspecific and CPSF (cleavage andpolyadenylation specificity factor)/AAUAAA-dependentpolyadenylation activity. This gene is located on chromosome 2 incontrast to the PAPII gene, which is located on chromosome 14.
Lee, Y.S., et al. J. Biol. Chem. 285(36):28105-28116(2010)Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)Kyriakopoulou, C.B., et al. J. Biol. Chem. 276(36):33504-33511(2001)Topalian, S.L., et al. Mol. Cell. Biol. 21(16):5614-5623(2001)Perumal, K., et al. J. Biol. Chem. 276(24):21791-21796(2001)
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