|Other Names||NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, CD314, KLRK1, D12S2489E, NKG2D|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13295a was selected from the N-term region of NKG2D. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.|
|Cellular Location||Cell membrane; Single- pass type II membrane protein Note=Colocalized with HCST on the cell surface|
|Tissue Location||Expressed in natural killer (NK) cells, CD8(+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs)|
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Provided below are standard protocols that you may find useful for product applications.
Natural killer (NK) cells are lymphocytes that can mediatelysis of certain tumor cells and virus-infected cells withoutprevious activation. They can also regulate specific humoral andcell-mediated immunity. NK cells preferentially express severalcalcium-dependent (C-type) lectins, which have been implicated inthe regulation of NK cell function. This gene encodes a member ofthe NKG2 family, and the encoded transmembrane protein ischaracterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 genefamily is located within the NK complex, a region that containsseveral C-type lectin genes preferentially expressed in NK cells.
Hanaoka, N., et al. J. Immunol. 185(10):5732-5742(2010)Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)Ma, J., et al. J. Med. Virol. 82(9):1501-1507(2010)Champsaur, M., et al. J. Immunol. 185(1):157-165(2010)Campbell, J.A., et al. J. Immunol. 185(1):28-32(2010)
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