|Other Names||WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2, WIPI2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13314a was selected from the N-term region of WIPI2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.|
|Cellular Location||Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.|
|Tissue Location||Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas Expression is down-regulated in pancreatic and in kidney tumors|
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Provided below are standard protocols that you may find useful for product applications.
WD40 repeat proteins are key components of many essentialbiologic functions. They regulate the assembly of multiproteincomplexes by presenting a beta-propeller platform for simultaneousand reversible protein-protein interactions. Members of the WIPIsubfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladedpropeller structure and contain a conserved motif for interactionwith phospholipids (Proikas-Cezanne et al., 2004 [PubMed15602573]).
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)Proikas-Cezanne, T., et al. Oncogene 23(58):9314-9325(2004)Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
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