|Other Names||Ragulator complex protein LAMTOR2, Endosomal adaptor protein p14, Late endosomal/lysosomal Mp1-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, Mitogen-activated protein-binding protein-interacting protein, MAPBP-interacting protein, Roadblock domain-containing protein 3, LAMTOR2, MAPBPIP, ROBLD3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13338c was selected from the Center region of ROBLD3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.|
|Cellular Location||Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side|
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Provided below are standard protocols that you may find useful for product applications.
The product of this gene is highly conserved with a mouseprotein associated with the cytoplasmic face of late endosomes andlysosomes. The mouse protein interacts with MAPK scaffold protein1, a component of the mitogen-activated protein kinase pathway. Inhumans, a mutation in this gene has been associated with a primaryimmunodeficiency syndrome, and suggests a role for this protein inendosomal biogenesis. Multiple transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].
Sancak, Y., et al. Cell 141(2):290-303(2010)Wunderlich, W., et al. J. Cell Biol. 152(4):765-776(2001)
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