DHRS4 Antibody (Center) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9BTZ2 |
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Clone Names | 90909040 |
Gene ID | 10901 |
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Other Names | Dehydrogenase/reductase SDR family member 4, NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase, CR, PHCR, NADPH-dependent retinol dehydrogenase/reductase, NRDR, humNRDR, Peroxisomal short-chain alcohol dehydrogenase, PSCD, SCAD-SRL, Short-chain dehydrogenase/reductase family member 4, DHRS4 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13614c was selected from the Center region of DHRS4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | DHRS4 (HGNC:16985) |
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Function | NADPH-dependent oxidoreductase which catalyzes the reduction of a variety of compounds bearing carbonyl groups including ketosteroids, alpha-dicarbonyl compounds, aldehydes, aromatic ketones and quinones (PubMed:18571493, PubMed:19056333). Reduces 3-ketosteroids and benzil into 3beta-hydroxysteroids and R-benzoin, respectively, in contrast to the stereoselectivity of non-primate DHRS4s which produce 3alpha-hydroxysteroids and S-benzoin (PubMed:19056333). Diplays low activity toward all-trans-retinal and no activity toward 9-cis-retinal as compared to non-primate mammals (PubMed:18571493, PubMed:19056333). In the reverse reaction, catalyze the NAD-dependent oxidation of 3beta- hydroxysteroids and alcohol, but with much lower efficiency (PubMed:18571493, PubMed:19056333). Involved in the metabolism of 3beta-hydroxysteroids, isatin and xenobiotic carbonyl compounds (PubMed:18571493, PubMed:19056333). |
Cellular Location | [Isoform 1]: Peroxisome Note=Isoform 4 is not peroxisomal. |
Tissue Location | [Isoform 1]: Predominantly expressed in normal cervix (at protein level). [Isoform 5]: Expressed in a few neoplastic cervical tissues. [Isoform 8]: High expression in liver. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DHRS4 reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones (By similarity).
References
Su, Z.J., et al. BMC Mol. Biol. 11, 43 (2010) :Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009) :Zhang, Q., et al. Biosci. Rep. 29(1):47-56(2009)Matsunaga, T., et al. Arch. Biochem. Biophys. 477(2):339-347(2008)Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008)
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