|Other Names||Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, SH2/SH3 adaptor protein NCK-alpha, NCK1, NCK|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13726a was selected from the N-term region of NCK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.|
|Cellular Location||Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is one of the signalingand transforming proteins containing Src homology 2 and 3 (SH2 andSH3) domains. It is located in the cytoplasm and is an adaptorprotein involved in transducing signals from receptor tyrosinekinases to downstream signal recipients such as RAS. Alternativelyspliced transcript variants encoding different isoforms have beenfound.
Barda-Saad, M., et al. EMBO J. 29(14):2315-2328(2010)Gehmlich, K., et al. Eur. J. Cell Biol. 89(5):351-364(2010)Preisinger, C., et al. Cell. Signal. 22(5):848-856(2010)Davila, S., et al. Genes Immun. 11(3):232-238(2010)Voss, M., et al. BMC Immunol. 10, 53 (2009) :
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