|Other Names||Receptor-interacting serine/threonine-protein kinase 2, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, RIP-2, Tyrosine-protein kinase RIPK2, RIPK2, CARDIAK, RICK, RIP2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13758a was selected from the N-term region of RIPK2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||CARDIAK, RICK, RIP2|
|Function||Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.|
|Tissue Location||Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node|
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This gene encodes a member of the receptor-interactingprotein (RIP) family of serine/threonine protein kinases. Theencoded protein contains a C-terminal caspase activation andrecruitment domain (CARD), and is a component of signalingcomplexes in both the innate and adaptive immune pathways. It is apotent activator of NF-kappaB and inducer of apoptosis in responseto various stimuli.
Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010)Du, X., et al. Mol. Cell. Biochem. 337 (1-2), 277-285 (2010) :Adams, S., et al. Exp. Cell Res. 316(5):728-736(2010)Zhang, F.R., et al. N. Engl. J. Med. 361(27):2609-2618(2009)Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
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