|Other Names||Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha, CBX5, HP1A|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13779c was selected from the Center region of CBX5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.|
|Cellular Location||Nucleus. Chromosome. Chromosome, centromere Note=Component of centromeric and pericentromeric heterochromatin Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase|
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This gene encodes a highly conserved nonhistone protein,which is a member of the heterochromatin protein family. Theprotein is enriched in the heterochromatin and associated withcentromeres. The protein has a single N-terminal chromodomain whichcan bind to histone proteins via methylated lysine residues, and aC-terminal chromo shadow-domain (CSD) which is responsible for thehomodimerization and interaction with a number ofchromatin-associated nonhistone proteins. The encoded product isinvolved in the formation of functional kinetochore throughinteraction with essential kinetochore proteins. The gene has apseudogene located on chromosome 3. Multiple alternatively splicedvariants, encoding the same protein, have been identified.
Nozawa, R.S., et al. Nat. Cell Biol. 12(7):719-727(2010)Zeng, W., et al. Epigenetics 5(4):287-292(2010)Emelyanov, A.V., et al. J. Biol. Chem. 285(20):15027-15037(2010)Kiyomitsu, T., et al. J. Cell Biol. 188(6):791-807(2010)Chaturvedi, P., et al. PLoS ONE 5 (5), E10620 (2010) :
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