|Other Names||FACT complex subunit SPT16, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT 140 kDa subunit, FACTp140, Facilitates chromatin transcription complex subunit SPT16, hSPT16, SUPT16H, FACT140, FACTP140|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13844c was selected from the Center region of SUPT16H. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).|
|Cellular Location||Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci|
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Provided below are standard protocols that you may find useful for product applications.
Transcription of protein-coding genes can be reconstitutedon naked DNA with only the general transcription factors and RNApolymerase II. However, this minimal system cannot transcribe DNApackaged into chromatin, indicating that accessory factors mayfacilitate access to DNA. One such factor, FACT (facilitateschromatin transcription), interacts specifically with histonesH2A/H2B to effect nucleosome disassembly and transcriptionelongation. FACT is composed of an 80 kDa subunit and a 140 kDasubunit; this gene encodes the 140 kDa subunit. [provided byRefSeq].
Heo, K., et al. Mol. Cell 30(1):86-97(2008)Tsuritani, K., et al. Genome Res. 17(7):1005-1014(2007)Li, Y., et al. J. Biol. Chem. 282(10):6936-6945(2007)Olsen, J.V., et al. Cell 127(3):635-648(2006)Olsen, J.V., et al. Cell 127(3):635-648(2006)
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