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TOP1 Antibody (N-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P11387 |
|---|---|
| Clone Names | 100430241 |
| Gene ID | 7150 |
|---|---|
| Other Names | DNA topoisomerase 1, DNA topoisomerase I, TOP1 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP13897a was selected from the N-term region of TOP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | TOP1 |
|---|---|
| Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)- enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter. |
| Cellular Location | Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli |
| Tissue Location | Endothelial cells.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a DNA topoisomerase, an enzyme thatcontrols and alters the topologic states of DNA duringtranscription. This enzyme catalyzes the transient breaking andrejoining of a single strand of DNA which allows the strands topass through one another, thus altering the topology of DNA. Thisgene is localized to chromosome 20 and has pseudogenes which resideon chromosomes 1 and 22.
References
Kjeldsen, E., et al. Anticancer Res. 30(9):3257-3265(2010)Reinhold, W.C., et al. Cancer Res. 70(6):2191-2203(2010)Tesauro, C., et al. Biochem. J. 425(3):531-539(2010)Ballot, C., et al. Mol. Cancer Ther. 8(12):3307-3317(2009)Lebedeva, N.A., et al. Biochemistry Mosc. 74(11):1278-1284(2009)
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