|Other Names||DNA topoisomerase 1, DNA topoisomerase I, TOP1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP13897a was selected from the N-term region of TOP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.|
|Cellular Location||Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli|
|Tissue Location||Endothelial cells.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a DNA topoisomerase, an enzyme thatcontrols and alters the topologic states of DNA duringtranscription. This enzyme catalyzes the transient breaking andrejoining of a single strand of DNA which allows the strands topass through one another, thus altering the topology of DNA. Thisgene is localized to chromosome 20 and has pseudogenes which resideon chromosomes 1 and 22.
Kjeldsen, E., et al. Anticancer Res. 30(9):3257-3265(2010)Reinhold, W.C., et al. Cancer Res. 70(6):2191-2203(2010)Tesauro, C., et al. Biochem. J. 425(3):531-539(2010)Ballot, C., et al. Mol. Cancer Ther. 8(12):3307-3317(2009)Lebedeva, N.A., et al. Biochemistry Mosc. 74(11):1278-1284(2009)
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