|Other Names||Serine/threonine-protein kinase PAK 7, p21-activated kinase 5, PAK-5, p21-activated kinase 7, PAK-7, Pak7, Pak5|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP14074a was selected from the N-term region of Mouse Pak7. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions (By similarity).|
|Cellular Location||Mitochondrion. Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival.|
|Tissue Location||Highly expressed in brain and eye. Also expressed in adrenal gland, pancreas, prostate and testes. Within the brain, expression is restricted to neurons. Present in brain but not in kidney, lung and spleen (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
The activated kinase acts on a variety of targets (By similarity).
Gobert, R.P., et al. Mol. Cell. Biol. 29(6):1538-1553(2009)Nekrasova, T., et al. Dev. Biol. 322(1):95-108(2008)Pagliarini, D.J., et al. Cell 134(1):112-123(2008)Sapir, T., et al. J. Neurosci. 28(22):5710-5720(2008)Trinidad, J.C., et al. Mol. Cell Proteomics 5(5):914-922(2006)
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