|Other Names||Poly(U)-binding-splicing factor PUF60, 60 kDa poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, FBP-interacting repressor, Ro-binding protein 1, RoBP1, Siah-binding protein 1, Siah-BP1, PUF60, FIR, ROBPI, SIAHBP1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||FIR, ROBPI, SIAHBP1|
|Function||DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.|
|Cellular Location||Nucleus. Note=Colocalizes partially with TROVE2|
|Tissue Location||Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.|
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The protein encoded by this gene is a Ro RNP-bindingprotein. It interacts with Ro RNPs and their interaction is thoughtto represent a gain of function for Ro RNPs. This protein alsoforms a ternary complex with far upstream element (FUSE) andFUSE-binding protein. It can repress a c-myc reporter via the FUSE.It is also known to target transcription factor IIH and inhibitactivated transcription. This gene is implicated in the xerodermapigmentosum disorder. There are two alternatively splicedtranscript variants of this gene encoding different isoforms. Thereseems to be evidence of multiple polyadenylation sites for thisgene.
Hsiao, H.H., et al. Biochemistry 49(22):4620-4634(2010)Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)Gao, J., et al. Genomics 91(4):347-355(2008)Hastings, M.L., et al. PLoS ONE 2 (6), E538 (2007) :Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
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