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PSMB8 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P28062 |
|---|---|
| Clone Names | 100507200 |
| Gene ID | 5696 |
|---|---|
| Other Names | Proteasome subunit beta type-8, Low molecular mass protein 7, Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13, Proteasome component C13, Proteasome subunit beta-5i, Really interesting new gene 10 protein, PSMB8, LMP7, PSMB5i, RING10, Y2 |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | PSMB8 |
|---|---|
| Synonyms | LMP7, PSMB5i, RING10, Y2 |
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Involved in the generation of spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein (PubMed:27049119). Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes. |
| Cellular Location | Cytoplasm {ECO:0000255|PROSITE-ProRule:PRU00809}. Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
The proteasome is a multicatalytic proteinase complex witha highly ordered ring-shaped 20S core structure. The core structureis composed of 4 rings of 28 non-identical subunits; 2 rings arecomposed of 7 alpha subunits and 2 rings are composed of 7 betasubunits. Proteasomes are distributed throughout eukaryotic cellsat a high concentration and cleave peptides in anATP/ubiquitin-dependent process in a non-lysosomal pathway. Anessential function of a modified proteasome, the immunoproteasome,is the processing of class I MHC peptides. This gene encodes amember of the proteasome B-type family, also known as the T1Bfamily, that is a 20S core beta subunit. This gene is located inthe class II region of the MHC (major histocompatibility complex).Expression of this gene is induced by gamma interferon and thisgene product replaces catalytic subunit 3 (proteasome beta 5subunit) in the immunoproteasome. Proteolytic processing isrequired to generate a mature subunit. Two alternative transcriptsencoding two isoforms have been identified; both isoforms areprocessed to yield the same mature subunit.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)Bravo, M.J., et al. Hum. Immunol. 71(7):708-711(2010)Shi, C., et al. Epidemiol. Infect., 1-9 (2010) In press :Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)Ustrell, V., et al. FEBS Lett. 376(3):155-158(1995)
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