|Other Names||Band 3 anion transport protein, Anion exchange protein 1, AE 1, Anion exchanger 1, Solute carrier family 4 member 1, CD233, SLC4A1, AE1, DI, EPB3|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||AE1, DI, EPB3|
|Function||Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Note=Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha- intercalated cells in the collecting duct in the kidney|
|Tissue Location||Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871)|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is part of the anionexchanger (AE) family and is expressed in the erythrocyte plasmamembrane, where it functions as a chloride/bicarbonate exchangerinvolved in carbon dioxide transport from tissues to lungs. Theprotein comprises two domains that are structurally andfunctionally distinct. The N-terminal 40kDa domain is located inthe cytoplasm and acts as an attachment site for the red cellskeleton by binding ankyrin. The glycosylated C-terminalmembrane-associated domain contains 12-14 membrane spanningsegments and carries out the stilbene disulphonate-sensitiveexchange transport of anions. The cytoplasmic tail at the extremeC-terminus of the membrane domain binds carbonic anhydrase II. Theencoded protein associates with the red cell membrane proteinglycophorin A and this association promotes the correct folding andtranslocation of the exchanger. This protein is predominantlydimeric but forms tetramers in the presence of ankyrin. Manymutations in this gene are known in man, and these mutations canlead to two types of disease: destabilization of red cell membraneleading to hereditary spherocytosis, and defective kidney acidsecretion leading to distal renal tubular acidosis. Other mutationsthat do not give rise to disease result in novel blood groupantigens, which form the Diego blood group system. Southeast Asianovalocytosis (SAO, Melanesian ovalocytosis) results from theheterozygous presence of a deletion in the encoded protein and iscommon in areas where Plasmodium falciparum malaria is endemic. Onenull mutation in this gene is known, resulting in very severeanemia and nephrocalcinosis.
Basu, A., et al. Biochemistry 49(43):9226-9240(2010)Sawasdee, N., et al. Biochem. Biophys. Res. Commun. 401(1):85-91(2010)Shmukler, B.E., et al. Am. J. Hematol. 85(10):824-828(2010)Novaretti, M.C., et al. Immunohematology 26(2):66-70(2010)Bruce, L.J., et al. Blood 101(10):4180-4188(2003)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.