|Other Names||Cyclin-dependent kinase 4, CRK3, Cell division protein kinase 4, PSK-J3, Cdk4, Crk3|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).|
|Cellular Location||Cytoplasm. Nucleus. Membrane. Note=Cytoplasmic when non- complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps Colocalizes with RB1 after release into the nucleus (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Cdk4 is probably involved in the control of the cell cycle.
Trivedi, C.M., et al. Dev. Cell 19(3):450-459(2010)Beilke, S., et al. Oncogene 29(28):4058-4067(2010)Puyol, M., et al. Cancer Cell 18(1):63-73(2010)Wiedemeyer, W.R., et al. Proc. Natl. Acad. Sci. U.S.A. 107(25):11501-11506(2010)Michaud, K., et al. Cancer Res. 70(8):3228-3238(2010)
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