|Other Names||Poly(ADP-ribose) glycohydrolase, PARG|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase (PubMed:23102699). PARG acts both as an endo- and exoglycosidase, releasing PAR of different length as well as ADP- ribose monomers (PubMed:23102699). Required for retinoid acid- dependent gene transactivation, probably by dePARsylating histone demethylase KDM4D, allowing chromatin derepression at RAR- dependent gene promoters (PubMed:23102699). Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).|
|Cellular Location||Isoform 1: Nucleus Note=Colocalizes with PCNA at replication foci. Relocalizes to the cytoplasm in response to DNA damage Isoform 3: Cytoplasm. Isoform 5: Mitochondrion matrix.|
|Tissue Location||Ubiquitously expressed.|
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Poly(ADP-ribose) glycohydrolase (PARG) is the majorenzyme responsible for the catabolism of poly(ADP-ribose), areversible covalent-modifier of chromosomal proteins. The proteinis found in many tissues and may be subject to proteolysisgenerating smaller, active products.
Whatcott, C.J., et al. Exp. Cell Res. 315(20):3477-3485(2009)Frizzell, K.M., et al. J. Biol. Chem. 284(49):33926-33938(2009)Erdelyi, K., et al. FASEB J. 23(10):3553-3563(2009)Ame, J.C., et al. J. Cell. Sci. 122 (PT 12), 1990-2002 (2009) :Uchiumi, F., et al. Genes Cells 13(12):1229-1247(2008)
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