|Other Names||Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2, Development and differentiation-enhancing factor 2, Paxillin-associated protein with ARF GAP activity 3, PAG3, Pyk2 C-terminus-associated protein, PAP, ASAP2, DDEF2, KIAA0400|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.|
|Cellular Location||Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Note=Colocalizes with F-actin and ARF6 in phagocytic cups|
|Tissue Location||Detected in heart, brain, placenta, kidney, monocytes and pancreas.|
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This gene encodes a multidomain protein containing anN-terminal alpha-helical region with a coiled-coil motif, followedby a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrinhomology region, a proline-rich region, and a C-terminal Srchomology 3 (SH3) domain. The protein localizes in the Golgiapparatus and at the plasma membrane, where it colocalizes withprotein tyrosine kinase 2-beta (PYK2). The encoded protein forms astable complex with PYK2 in vivo. This interaction appears to bemediated by binding of its SH3 domain to the C-terminalproline-rich domain of PYK2. The encoded protein is tyrosinephosphorylated by activated PYK2. It has catalytic activity forclass I and II ArfGAPs in vitro, and can bind the class III ArfARF6 without immediate GAP activity. The encoded protein isbelieved to function as an ARF GAP that controls ARF-mediatedvesicle budding when recruited to Golgi membranes. In addition, itfunctions as a substrate and downstream target for PYK2 and SRC, apathway that may be involved in the regulation of vesiculartransport. Multiple transcript variants encoding different isoformshave been found for this gene.
Baranzini, S.E., et al. Hum. Mol. Genet. 18(4):767-778(2009)Wu, C., et al. Proteomics 7(11):1775-1785(2007)Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :Olsen, J.V., et al. Cell 127(3):635-648(2006)
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