|Other Names||Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, Ubiquitin carrier protein T, Ubiquitin-protein ligase T, UBE2T|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys- 63'-linked polyubiquitination (PubMed:20061386).|
|Cellular Location||Nucleus. Note=Accumulates to chromatin|
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Provided below are standard protocols that you may find useful for product applications.
The covalent conjugation of ubiquitin to proteinsregulates diverse cellular pathways and proteins. Ubiquitin istransferred to a target protein through a concerted action of aubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme(E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al.,2006 [PubMed 16916645]).
Ueki, T., et al. Cancer Res. 69(22):8752-8760(2009)Longerich, S., et al. J. Biol. Chem. 284(35):23182-23186(2009)Alpi, A.F., et al. Mol. Cell 32(6):767-777(2008)Hao, J., et al. Tumour Biol. 29(3):195-203(2008)Machida, Y.J., et al. Mol. Cell 23(4):589-596(2006)
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