|Other Names||Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Oligosaccharyl transferase subunit DAD1, Defender against cell death 1, DAD-1, DAD1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis (By similarity).|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
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DAD1, the defender against apoptotic cell death, wasinitially identified as a negative regulator of programmed celldeath in the temperature sensitive tsBN7 cell line. The DAD1protein disappeared in temperature-sensitive cells following ashift to the nonpermissive temperature, suggesting that loss of theDAD1 protein triggered apoptosis. DAD1 is believed to be a tightlyassociated subunit of oligosaccharyltransferase both in the intactmembrane and in the purified enzyme, thus reflecting the essentialnature of N-linked glycosylation in eukaryotes. [provided byRefSeq].
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Kulke, M.H., et al. Genes Chromosomes Cancer 47(7):591-603(2008)Shibatani, T., et al. Biochemistry 44(16):5982-5992(2005)
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