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QTRT1 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
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- BACKGROUND
| Primary Accession | Q9BXR0 |
|---|---|
| Clone Names | 110726177 |
| Gene ID | 81890 |
|---|---|
| Other Names | Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase, QTRT1, TGT, TGUT |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | QTRT1 {ECO:0000255|HAMAP-Rule:MF_03218} |
|---|---|
| Synonyms | TGT, TGUT |
| Function | Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154, PubMed:34009357, PubMed:34241577). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity). Modification of cytoplasmic tRNAs with queuosine controls the elongation speed of cognate codons, thereby ensuring the correct folding of nascent proteins to maintain proteome integrity (PubMed:30093495). |
| Cellular Location | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03218}. Mitochondrion outer membrane {ECO:0000255|HAMAP-Rule:MF_03218}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_03218}; Cytoplasmic side {ECO:0000255|HAMAP-Rule:MF_03218}. Note=Weakly associates with mitochondria, possibly via QTRT2. {ECO:0000255|HAMAP- Rule:MF_03218} |
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Provided below are standard protocols that you may find useful for product applications.
Background
tRNA-guanine transglycosylase (TGT; EC 2.4.2.29)synthesizes queuosine (Q), which is found in tRNAs that recognizeNAU and NAC codons, encoding tyr, asn, asp, and his. ProkaryoticTGT is a single protein of 43 kD. In contrast, mammalian TGTappears to be a heterodimer consisting of a 60-kD subunit (USP14;MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande andKatze, 2001 [PubMed 11255023]).
References
Chen, Y.C., et al. RNA 16(5):958-968(2010)Deshpande, K.L., et al. Gene 265 (1-2), 205-212 (2001) :
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