|Other Names||Queuine tRNA-ribosyltransferase, Guanine insertion enzyme, tRNA-guanine transglycosylase, QTRT1, TGT, TGUT|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Interacts with QTRTD1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine) (By similarity).|
|Cellular Location||Cytoplasm. Mitochondrion. Nucleus. Note=Weakly associates with mitochondria, possibly via QTRTD1.|
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Provided below are standard protocols that you may find useful for product applications.
tRNA-guanine transglycosylase (TGT; EC 126.96.36.199)synthesizes queuosine (Q), which is found in tRNAs that recognizeNAU and NAC codons, encoding tyr, asn, asp, and his. ProkaryoticTGT is a single protein of 43 kD. In contrast, mammalian TGTappears to be a heterodimer consisting of a 60-kD subunit (USP14;MIM 607274) and a 43-kD catalytic subunit (QTRT1) (Deshpande andKatze, 2001 [PubMed 11255023]).
Chen, Y.C., et al. RNA 16(5):958-968(2010)Deshpande, K.L., et al. Gene 265 (1-2), 205-212 (2001) :
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