|Other Names||Neutral ceramidase, N-CDase, NCDase, Acylsphingosine deacylase 2, BCDase, LCDase, hCD, N-acylsphingosine amidohydrolase 2, Non-lysosomal ceramidase, Neutral ceramidase soluble form, ASAH2, HNAC1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo|
|Tissue Location||Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B.|
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Provided below are standard protocols that you may find useful for product applications.
Ceramidases (EC 220.127.116.11), such as ASAH2, catalyzehydrolysis of the N-acyl linkage of ceramide, a second messenger ina variety of cellular events, to produce sphingosine. Sphingosineexerts both mitogenic and apoptosis-inducing activities, and itsphosphorylated form functions as an intra- and intercellular secondmessenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed11328816]).
Uchida, Y., et al. J. Invest. Dermatol. 130(10):2472-2480(2010)Hong, K.K., et al. J. Korean Med. Sci. 22(5):862-867(2007)Ohlsson, L., et al. Biochimie 89(8):950-960(2007)Avramopoulos, D., et al. Neurogenetics 8(2):111-120(2007)Galadari, S., et al. Biochem. J. 393 (PT 3), 687-695 (2006) :
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