|Other Names||Alkaline ceramidase 1, AlkCDase 1, Alkaline CDase 1, Acylsphingosine deacylase 3, N-acylsphingosine amidohydrolase 3, ACER1, ASAH3|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 8.0. Has a highly restricted substrate specificity for the natural stereoisomer of ceramide with D-erythro-sphingosine but not D-ribo- phytosphingosine or D-erythro-dihydrosphingosine as a backbone. May have a role in regulating the levels of bioactive lipids ceramide and sphingosine 1-phosphate, as well as complex sphingolipids (By similarity).|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Mainly expressed in epidermis.|
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Ceramides are synthesized during epidermal differentiationand accumulate within the interstices of the stratum corneum, wherethey represent critical components of the epidermal permeabilitybarrier. Excess cellular ceramide can trigger antimitogenic signalsand induce apoptosis, and the ceramide metabolites sphingosine andsphingosine-1-phosphate (S1P) are important bioregulatorymolecules. Ceramide hydrolysis in the nucleated cell layersregulates keratinocyte proliferation and apoptosis in response toexternal stress. Ceramide hydrolysis also occurs at the stratumcorneum, releasing free sphingoid base that functions as anendogenous antimicrobial agent. ACER1 is highly expressed inepidermis and catalyzes the hydrolysis of very long chain ceramidesto generate sphingosine (Houben et al., 2006 [PubMed 16477081]; Sunet al., 2008 [PubMed 17713573]).
Sun, W., et al. J. Invest. Dermatol. 128(2):389-397(2008)Toulza, E., et al. Genome Biol. 8 (6), R107 (2007) :Houben, E., et al. J. Lipid Res. 47(5):1063-1070(2006)Mao, C., et al. J. Biol. Chem. 278(33):31184-31191(2003)Ito, M., et al. Tanpakushitsu Kakusan Koso 47 (4 SUPPL), 455-462 (2002) :
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