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MKL1 Antibody (Center) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q969V6 |
|---|---|
| Clone Names | 100623022 |
| Gene ID | 57591 |
|---|---|
| Other Names | MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein, Myocardin-related transcription factor A, MRTF-A, MKL1, KIAA1438, MAL |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | MRTFA (HGNC:14334) |
|---|---|
| Function | Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:26224645). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G- actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex. Activity is also regulated by filamentous actin (F-actin) in the nucleus. |
| Cellular Location | Cytoplasm. Nucleus Note=Subcellular location is tightly regulated by actin both in cytoplasm and nucleus: high levels of G-actin in the nucleus observed during serum deprivation lead to low levels of nuclear MRTFA, while reduced levels of nuclear G-actin result in accumulation of MRTFA in the nucleus (By similarity). G-actin-binding in the cytoplasm inhibits nuclear import by masking the nuclear localization signal (NLS) (By similarity). In contrast, binding to nuclear globular actin (G-actin) promotes nuclear export to the cytoplasm (By similarity). Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear actin, which decreases nuclear G-actin pool, thereby promoting retention of MRTFA in the nucleus and subsequent formation of an active complex with SRF (PubMed:24440334). {ECO:0000250|UniProtKB:Q8K4J6, ECO:0000269|PubMed:24440334} |
| Tissue Location | Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain (PubMed:11344311). Expressed in peripheral blood mononuclear cells (at protein level) (PubMed:26224645) |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene interacts with thetranscription factor myocardin, a key regulator of smooth musclecell differentiation. The encoded protein is predominantly nuclearand may help transduce signals from the cytoskeleton to thenucleus. This gene is involved in a specific translocation eventthat creates a fusion of this gene and the RNA-binding motifprotein-15 gene. This translocation has been associated with acutemegakaryocytic leukemia.
References
Gilles, L., et al. Blood 114(19):4221-4232(2009)Hinohara, K., et al. Hum. Genet. 126(4):539-547(2009)Estrada, K., et al. Hum. Mol. Genet. 18(18):3516-3524(2009)Brandt, D.T., et al. Nat. Cell Biol. 11(5):557-568(2009)Medjkane, S., et al. Nat. Cell Biol. 11(3):257-268(2009)
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