|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP1964a was selected from the N-term region of human Rde-1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Double-stranded (ds) RNA is a potent sequence-specific inhibitor of gene function. RNAi (RNA interference) is a multistep process involving several proteins and small RNAs 21-25 nucleotdies in length(called siRNAs) derived from cleavage of the dsRNA trigger. Genetic studies have implicated several RNA interference-deficient (rde) family members in germline maintenance and development, and several simple loss of function mutants have been identified. Family members rde-1 and rde-4 are required for RNAi but are not essential for organismal viability. While rde-1 and rde-4 are distinct from other RNAi-deficient family members both both for their inability to mobilize transposons and lack of chromosome loss, each appears to have a different role in the interference mechanism. Evidence indicates that rde-4 is involved before or during production of siRNAs, whereas rde-1 acts after the siRNAs have been formed.
Tabara H, et al. Cell. 2002. 109(7):861-71.Parrish S,et al. RNA. 2001. 7(10):1397-402.Fagard M, et al. PNAS. 2000. 97(21):11650-4 Ketting RF, et al. Nature. 2000. 404(6775):296-8.Tabara H, et al. Cell. 1999. 99(2):123-32.
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