Ribophorin (RPN1) Antibody (C-term) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P04843 |
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Clone Names | 3102207 |
Gene ID | 6184 |
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Other Names | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1, RPN1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2409b was selected from the C-term region of human RPN1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | RPN1 (HGNC:10381) |
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Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
Cellular Location | Endoplasmic reticulum {ECO:0000250|UniProtKB:E2RQ08, ECO:0000250|UniProtKB:Q9GMB0} Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
Tissue Location | Expressed in all tissues tested. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ribophorins 1 and 2 are abundant and highly conserved glycoproteins residing in the endoplasic reticulum, that participate in ribosome binding. Mammalian oligosaccharyltransferase activity is associated with a protein complex composed of RPN1, RPN2, and an oligosaccharyltransferase protein. RPN1 is a component of the proteasome base. The ubiquitin-like (UBL) domain of recombinant Rad23 interacts with proteasomes through the leucine-rich repeat domain of RPN1. The RPN1 gene maps to chromosome 3 in somatic cell hybrids, and the RPN2 gene maps to chromosome 20 by in situ hybridization.
References
Fu, J., et al., J. Biol. Chem. 275(6):3984-3990 (2000).Pekarsky, Y., et al., Cancer Res. 57(18):3914-3919 (1997).Crimaudo, C., et al., EMBO J. 6(1):75-82 (1987).
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