|Other Names||Long-chain-fatty-acid--CoA ligase 5, Long-chain acyl-CoA synthetase 5, LACS 5, ACSL5, ACS5, FACL5|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2536a was selected from the N-term region of human FACL5 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells.|
|Cellular Location||Mitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Long chain acyl-CoA synthetase (LACS), or long chain fatty acid-CoA ligases (FACLs), convert free long chain fatty acids into fatty acyl-CoA esters, which are key intermediates in the synthesis of complex lipids. The deduced 683-amino acid FACL5 protein shares approximately 80% amino acid identity with the rat sequence. Northern blot analysis detected major FACL5 transcripts of 2.5 and 3.7 kb in a many tissues, with highest expression in uterus and spleen. Markedly increased levels of FACL5 transcripts are detected in a glioma line and in primary gliomas of grade IV malignancy, Cultured glioma cells infected with an adenovirus encoding FACL5 displayed induced cell growth on exposure to palmitate. These results suggest a novel fatty acid-induced glioma cell growth mediated by FACL5.
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