|Other Names||Long-chain-fatty-acid--CoA ligase 5, Long-chain acyl-CoA synthetase 5, LACS 5, ACSL5, ACS5, FACL5|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2536a was selected from the N-term region of human FACL5 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells.|
|Cellular Location||Mitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Long chain acyl-CoA synthetase (LACS), or long chain fatty acid-CoA ligases (FACLs), convert free long chain fatty acids into fatty acyl-CoA esters, which are key intermediates in the synthesis of complex lipids. The deduced 683-amino acid FACL5 protein shares approximately 80% amino acid identity with the rat sequence. Northern blot analysis detected major FACL5 transcripts of 2.5 and 3.7 kb in a many tissues, with highest expression in uterus and spleen. Markedly increased levels of FACL5 transcripts are detected in a glioma line and in primary gliomas of grade IV malignancy, Cultured glioma cells infected with an adenovirus encoding FACL5 displayed induced cell growth on exposure to palmitate. These results suggest a novel fatty acid-induced glioma cell growth mediated by FACL5.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.