|Other Names||Sterol 26-hydroxylase, mitochondrial, 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase, Cytochrome P-450C27/25, Cytochrome P450 27, Sterol 27-hydroxylase, Vitamin D(3) 25-hydroxylase, CYP27A1, CYP27|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2853a was selected from the N-term region of human CYP27A1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta- cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3- 25-hydroxylase activity.|
|Cellular Location||Mitochondrion membrane.|
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Provided below are standard protocols that you may find useful for product applications.
CYP27A1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in CYP27A1 gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease.
Cali J.J., Russell D.W.J. Biol. Chem. 266:7774-7778(1991)Guo Y.-D., Strugnell S., Back D.W., Jones G.Proc. Natl. Acad. Sci. U.S.A. 90:8668-8672(1993)Chen W., Kubota S., Kim K.-S., Cheng J., Kuriyama M.,J. Lipid Res. 38:870-879(1997) Chen W., Kubota S., Ujike H., Ishihara T.Biochemistry 37:15050-15056(1998)
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