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|Other Names||14-3-3 protein epsilon, 14-3-3E, YWHAE|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP3001a was selected from the region of human Phospho-14-3-3-T232. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Function||Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.|
|Cellular Location||Cytoplasm. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
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The 14-3-3 family of proteins mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
Urschel, S., et al., J. Biol. Chem. 280(17):16987-16993 (2005).Yanagi, M., et al., J. Hum. Genet. 50(4):210-216 (2005).Wang, X., et al., J. Biol. Chem. 279(47):49460-49469 (2004).Clark, K.L., et al., J. Biol. Chem. 279(19):19401-19406 (2004).Wang, X., et al., Mol. Cell. Biol. 23(17):6013-6026 (2003).
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