|Other Names||Twinfilin-1, Protein A6, Protein tyrosine kinase 9, TWF1, PTK9|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP3545a was selected from the region of human Phospho-TWF1-pY321. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Note=Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts (By similarity).|
|Tissue Location||Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver|
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Provided below are standard protocols that you may find useful for product applications.
Twinfilin is an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
Vartiainen, M.K., et al., J. Biol. Chem. 278(36):34347-34355 (2003).Ojala, P.J., et al., Mol. Biol. Cell 13(11):3811-3821 (2002).Beeler, J.F., et al., Mol. Cell. Biol. 14(2):982-988 (1994).Palmgren, S., et al., J. Cell. Sci. 115 (Pt 5), 881-886 (2002)
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