|Other Names||Vimentin, VIM|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP3546a was selected from the region of human Phospho-Vimentin-S82. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.|
|Tissue Location||Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
Whipple,R.A., Cancer Res. 68 (14), 5678-5688 (2008)Garcia-Verdugo,I., Biochemistry 47 (18), 5127-5138 (2008)
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