|Other Names||5' exonuclease Apollo, 31--, DNA cross-link repair 1B protein, SNM1 homolog B, SNMIB, hSNM1B, DCLRE1B, SNM1B|
|Target/Specificity||The synthetic peptide sequence is selected from aa 223-236 of HUMAN DCLRE1B|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)- mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint.|
|Cellular Location||Chromosome, telomere. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Note=Mainly localizes to telomeres, recruited via its interaction with TERF2. During mitosis, localizes to the centrosome|
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Provided below are standard protocols that you may find useful for product applications.
DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]).
Anders, M., et al. Cell Cycle 8(11):1725-1732(2009) Liu, L., et al. Cell Cycle 8(4):628-638(2009) Freibaum, B.D., et al. J. Biol. Chem. 283(35):23671-23676(2008) Bae, J.B., et al. Oncogene 27(37):5045-5056(2008) Demuth, I., et al. DNA Repair (Amst.) 7(8):1192-1201(2008) Matsuoka, S., et al. Science 316(5828):1160-1166(2007) Lenain, C., et al. Curr. Biol. 16(13):1303-1310(2006) Freibaum, B.D., et al. J. Biol. Chem. 281(22):15033-15036(2006) Ishiai, M., et al. Mol. Cell. Biol. 24(24):10733-10741(2004) Demuth, I., et al. Oncogene 23(53):8611-8618(2004) Dronkert, M.L., et al. Mol. Cell. Biol. 20(13):4553-4561(2000)
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