|Other Names||Non-structural protein 3b, ns3b, Accessory protein 3b, Protein X2, 3b|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6002a was selected from the N-term region of human SARS virus PUP2 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Induces host cell G0/G1 arrest and apoptosis.|
|Cellular Location||Host nucleus, host nucleolus. Host mitochondrion EMBL; AY278741; AAP13447.1; -; Genomic_RNA EMBL; AY274119; AAP41039.1; -; Genomic_RNA EMBL; AY282752; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY278554; AAP13569.1; -; Genomic_RNA EMBL; AY278491; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY278487; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY278488; AAP30032.1; -; Genomic_RNA EMBL; AY278489; AAP51229.1; -; Genomic_RNA EMBL; AY278490; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY279354; -; NOT_ANNOTATED_CDS; Genomic_RNA EMBL; AY291451; AAP37019.1; -; Genomic_RNA EMBL; AY310120; AAP50487.1; -; Genomic_RNA EMBL; AY291315; AAP33699.1; -; Genomic_RNA EMBL; AY463059; AAR86789.1; -; Genomic_RNA EMBL; AY338174; AAQ01599.1; -; Genomic_RNA EMBL; AY338175; AAQ01611.1; -; Genomic_RNA EMBL; AY348314; AAP97884.1; -; Genomic_RNA EMBL; AP006557; BAC81350.1; -; Genomic_RNA EMBL; AP006558; BAC81364.1; -; Genomic_RNA EMBL; AP006559; BAC81378.1; -; Genomic_RNA EMBL; AP006560; BAC81392.1; -; Genomic_RNA EMBL; AP006561; BAC81406.1; -; Genomic_RNA EMBL; AY323977; AAP72976.1; -; Genomic_RNA EMBL; AY427439; AAQ94062.1; -; Genomic_RNA RefSeq; NP_828853.1; NC_004718.3 GeneID; 1489670; - KEGG; vg:1489670; - Proteomes; UP000000354; Genome GO; GO:0033650; C:host cell mitochondrion; IEA:UniProtKB-SubCell GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell GO; GO:0039526; P:modulation by virus of host apoptotic process; IEA:UniProtKB-KW GO; GO:0039646; P:modulation by virus of host G0/G1 transition checkpoint; IEA:UniProtKB-KW GO; GO:0001817; P:regulation of cytokine production; IEP:CACAO InterPro; IPR022117; SARS_3b Pfam; PF12383; SARS_3b; 1 1: Evidence at protein level; Apoptosis; Complete proteome; G0/G1 host cell cycle checkpoint dysregulation by virus; Host mitochondrion; Host nucleus; Host-virus interaction; Modulation of host cell apoptosis by virus; Modulation of host cell cycle by virus; Reference proteome CHAIN 1 154 Non-structural protein 3b /FTId=PRO_0000106132 REGION 80 138 Mitochondrial targeting signal REGION 134 154 Nucleolar targeting MOTIF 135 153 Bipartite nuclear localization signal VARIANT 31 31 K -> Q (in strain: Isolate GD01) VARIANT 99 99 T -> I (in strain: Isolate BJ02) VARIANT 115 115 L -> Q (in strain: Isolate GD01) VARIANT 121 121 Q -> P (in strain: Isolate BJ01 and Isolate BJ04) SEQUENCE 154 AA; 17750 MW; 82AB0B68F11060A8 CRC64; MMPTTLFAGT HITMTTVYHI TVSQIQLSLL KVTAFQHQNS KKTTKLVVIL RIGTQVLKTM SLYMAISPKF TTSLSLHKLL QTLVLKMLHS SSLTSLLKTH RMCKYTQSTA LQELLIQQWI QFMMSRRRLL ACLCKHKKVS TNLCTHSFRK KQVR|
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Provided below are standard protocols that you may find useful for product applications.
The SARS-CoV genome contains five major open reading frames (ORFs) that encode the replicase polyprotein (R), the spike (S), envelope (E), and membrane (M) glycoproteins; and the nucleocapsid protein (N). Other proteins not falling into these categories have been termed PUPs (putative uncharacterized proteins) for their unknown structural or functional features and dissimilarity to those known sequences. However, it has been found that some of the PUPs matched the entries in the NCBI database.PUP2 has a counterpart in Isolate Tor2, the ORF4. It matches 4 segments of different entries in GenBank: 138 amino acids with NADH dehydrogenase subunit2 of Laudakia stoliczkana, 137 amino acids with a hypothetical protein of Methanosarcina barkeri, 85 amino acids with myosin IXb of Homo sapiens, and 85 amino acids with MY9B HUMAN myosin IXb. All of these alignments are 28% identical.
He, R., et al., Biochem. Biophys. Res. Commun. 316(2):476-483 (2004).Snijder, E.J., et al., J. Mol. Biol. 331(5):991-1004 (2003).Marra, M.A., et al., Science 300(5624):1399-1404 (2003).
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