|Other Names||Melanoma-associated antigen D1, MAGE tumor antigen CCF, MAGE-D1 antigen, Neurotrophin receptor-interacting MAGE homolog, MAGED1, NRAGE|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6176a was selected from the Center region of human MAGED1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. Note=Expression shifts from the cytoplasm to the plasma membrane upon stimulation with NGF.|
|Tissue Location||Expressed in bone marrow stromal cells from both multiple myeloma patients and healthy donors. Seems to be ubiquitously expressed|
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Provided below are standard protocols that you may find useful for product applications.
MAGED1 is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway.
Sasaki, A., et al., J. Biol. Chem. 277(25):22541-22546 (2002).Kubu, C.J., et al., Genomics 70(1):150-152 (2000).Salehi, A.H., et al., Neuron 27(2):279-288 (2000).Pold, M., et al., Genomics 59(2):161-167 (1999).
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