|Other Names||Forkhead box protein O4, Fork head domain transcription factor AFX1, FOXO4, AFX, AFX1, MLLT7|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6193a was selected from the N-term region of human MLLT7 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||AFX, AFX1, MLLT7|
|Function||Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.|
|Cellular Location||Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm Dephosphorylation triggers nuclear translocation Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm|
|Tissue Location||Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas|
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The MLLT7 (Mixed-lineage leukemia translocated to 7) protein is a forkhead transcription factor that activates apoptosis by inducing the BCL-6 transcriptional repressor. MLL-MLLT7 fusion protein transformw myeloid progenitors and impairs forkhead protein function. MLLT7 is a target of the phosphatidylinositol 3-kinase/PKB insulin signaling pathway and the AMP-activated protein kinase-dependent pathway.
Crossley, L.J., J. Leukoc. Biol. 74(4):583-592 (2003).Tang, T.T., et al., J. Biol. Chem. 278(32):30125-30135 (2003).So, C.W., et al., Mol. Cell. Biol. 22(18):6542-6552 (2002).Tang, T.T., et al., J. Biol. Chem. 277(16):14255-14265 (2002).Kops, G.J., et al., Mol. Cell. Biol. 22(7):2025-2036 (2002).
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