|Other Names||2'-5'-oligoadenylate synthase 1, (2-5')oligo(A) synthase 1, 2-5A synthase 1, E18/E16, p46/p42 OAS, OAS1, OIAS|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6226a was selected from the C-term region of human OAS1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.|
|Cellular Location||Cytoplasm. Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Secreted Note=Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions|
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Provided below are standard protocols that you may find useful for product applications.
OAS1 is an interferon inducible protein that may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. It binds double-stranded RNA and polymerizes ATP into PPP(A2'P5'A)N oligomers, which activate the latent RNase L that, when activated, cleaves single-stranded RNAs. This protein is associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).Sarkar, S.N., et al., J. Biol. Chem. 274(36):25535-25542 (1999).Ghosh, A., et al., J. Biol. Chem. 272(52):33220-33226 (1997).Ghosh, S.K., et al., J. Biol. Chem. 266(23):15293-15299 (1991).Rutherford, M.N., et al., EMBO J. 7(3):751-759 (1988).
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