|Other Names||M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6272d was selected from the S78 region of human Cdc25A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.|
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Provided below are standard protocols that you may find useful for product applications.
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.
Ito, Y., et al., Int. J. Mol. Med. 13(3):431-435 (2004).Nemoto, K., et al., Prostate 58(1):95-102 (2004).Goloudina, A., et al., Cell Cycle 2(5):473-478 (2003).Chen, M.S., et al., Mol. Cell. Biol. 23(21):7488-7497 (2003).Chow, J.P., et al., Mol. Biol. Cell 14(10):3989-4002 (2003).
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